two-compartment boyden chamber matrigel invasion assay Search Results


two-compartment boyden chamber matrigel invasion assay  (Corning Life Sciences)
90
Corning Life Sciences two-compartment boyden chamber matrigel invasion assay
Effect of DN-LRP5 on PC-3 cells invasion, colony formation, and migration in vitro . Control (PC-3-pcDNA3.1) and experimental (PC-3-DN-LRP5) cells invasive capacity was evaluated by using a <t>Boyden</t> chamber <t>Matrigel</t> invasion assay. After 18 h of incubation, the invaded cells were fixed, stained, and 10 random fields were counted. Number of cells invading is shown as bar diagram ± SEM (A) as described in “Material and Methods”. Using soft agar assay, the ability of control and experimental cells to form colonies was examined and the number of colonies formed in each group was counted (B) as described in “Material and Methods”. PC-3 cells migration was determined by wound healing assay by seeding PC-3-pcDNA3.1 and PC-3-DN-LRPP5 cells in six-well plates and allowing them to grow as a monolayer and making a wound as described in “Material and Methods”. Control and experimental migrating cells were photographed at different time points (C). Percent wound healing was recorded at different time points, and percentage of wound healing with respect to T 0 was calculated using the equation described in “Material and Methods”. Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control is represented by an asterisk ( P < 0.05).
Two Compartment Boyden Chamber Matrigel Invasion Assay, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-compartment+boyden+chamber+matrigel+invasion+assay/two+compartment+boyden+chamber+matrigel+invasion+assay/pmc03892794-52-15-21
Average 90 stars, based on 1 article reviews
two-compartment boyden chamber matrigel invasion assay - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Effect of DN-LRP5 on PC-3 cells invasion, colony formation, and migration in vitro . Control (PC-3-pcDNA3.1) and experimental (PC-3-DN-LRP5) cells invasive capacity was evaluated by using a Boyden chamber Matrigel invasion assay. After 18 h of incubation, the invaded cells were fixed, stained, and 10 random fields were counted. Number of cells invading is shown as bar diagram ± SEM (A) as described in “Material and Methods”. Using soft agar assay, the ability of control and experimental cells to form colonies was examined and the number of colonies formed in each group was counted (B) as described in “Material and Methods”. PC-3 cells migration was determined by wound healing assay by seeding PC-3-pcDNA3.1 and PC-3-DN-LRPP5 cells in six-well plates and allowing them to grow as a monolayer and making a wound as described in “Material and Methods”. Control and experimental migrating cells were photographed at different time points (C). Percent wound healing was recorded at different time points, and percentage of wound healing with respect to T 0 was calculated using the equation described in “Material and Methods”. Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control is represented by an asterisk ( P < 0.05).

Journal: Cancer Medicine

Article Title: LRP5 knockdown: effect on prostate cancer invasion growth and skeletal metastasis in vitro and in vivo

doi: 10.1002/cam4.111

Figure Lengend Snippet: Effect of DN-LRP5 on PC-3 cells invasion, colony formation, and migration in vitro . Control (PC-3-pcDNA3.1) and experimental (PC-3-DN-LRP5) cells invasive capacity was evaluated by using a Boyden chamber Matrigel invasion assay. After 18 h of incubation, the invaded cells were fixed, stained, and 10 random fields were counted. Number of cells invading is shown as bar diagram ± SEM (A) as described in “Material and Methods”. Using soft agar assay, the ability of control and experimental cells to form colonies was examined and the number of colonies formed in each group was counted (B) as described in “Material and Methods”. PC-3 cells migration was determined by wound healing assay by seeding PC-3-pcDNA3.1 and PC-3-DN-LRPP5 cells in six-well plates and allowing them to grow as a monolayer and making a wound as described in “Material and Methods”. Control and experimental migrating cells were photographed at different time points (C). Percent wound healing was recorded at different time points, and percentage of wound healing with respect to T 0 was calculated using the equation described in “Material and Methods”. Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control is represented by an asterisk ( P < 0.05).

Article Snippet: The invasive capacity of 5 × 10 4 PC-3-pcDNA3.1 and PC-3-DN-LRP5 cells were examined using two-compartment Boyden chamber Matrigel invasion assay (Costar Transwell, Corning Corporation, Corning, NY) as described previously .

Techniques: Migration, In Vitro, Invasion Assay, Incubation, Staining, Soft Agar Assay, Wound Healing Assay